{"created":"2023-05-15T12:25:48.472874+00:00","id":1122,"links":{},"metadata":{"_buckets":{"deposit":"941cde94-fb2f-423d-9a94-83d67b45fe6f"},"_deposit":{"created_by":4,"id":"1122","owners":[4],"pid":{"revision_id":0,"type":"depid","value":"1122"},"status":"published"},"_oai":{"id":"oai:kait.repo.nii.ac.jp:00001122","sets":["2:16:42:129"]},"author_link":[],"item_10002_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2015-03-20","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"12","bibliographicPageStart":"9","bibliographicVolumeNumber":"39","bibliographic_titles":[{"bibliographic_title":"神奈川工科大学研究報告.B,理工学編"}]}]},"item_10002_description_19":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_10002_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Chaperonin GroEL is double ring structure which constructed with 14-mer of 57 kDa subunits. GroEL has two cavities to trap both newly polypeptides and denatured proteins, and folds them with co-chaperonin GroES and ATP. Both Asp-52 and Asp-398 in E. coli GroEL are important for ATP hydrolysis. The ATPase activity of GroEL(D52A/D398A) mutants was <0.01 % of wild-type GroEL. GroEL(D52A/D398A) formed a extremely stable symmetric football-shaped GroEL-GroES complex in the presence of ATP, with a half-time of ~150 h (~6 days). We applied GroEL(D52A/D398A) as a nano-sized capsule that could encapsulate metal nanoparticles in its two cavities, and tried to align GroEL(D52A/D398A) complexes encapsulating metal nano particles on the surface of a TEM grid.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10002_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.34411/00001115","subitem_identifier_reg_type":"JaLC"}]},"item_10002_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"神奈川工科大学"}]},"item_10002_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA12669200","subitem_source_identifier_type":"NCID"}]},"item_10002_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"21882878","subitem_source_identifier_type":"PISSN"}]},"item_10002_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"依田, ひろみ","creatorNameLang":"ja"}]},{"creatorNames":[{"creatorName":"小池, あゆみ","creatorNameLang":"ja"}]},{"creatorNames":[{"creatorName":"Yoda, Hiromi","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Koike-Takeshita, Ayumi","creatorNameLang":"en"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2015-04-30"}],"displaytype":"detail","filename":"kkb-039-003.pdf","filesize":[{"value":"1.3 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"kkb-039-003.pdf","objectType":"fulltext","url":"https://kait.repo.nii.ac.jp/record/1122/files/kkb-039-003.pdf"},"version_id":"1e964a68-cf19-47c2-b8e5-5ab24e99a806"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"GroEL","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"GroES","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Chaperonin complex","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"metal nano particles","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"金属ナノ粒子内包 GroEL 複合体整列の検討 -- 分析電子顕微鏡システム利用研究成果、そのXXV(2) --","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"金属ナノ粒子内包 GroEL 複合体整列の検討 -- 分析電子顕微鏡システム利用研究成果、そのXXV(2) --","subitem_title_language":"ja"},{"subitem_title":"Approach for an alignment of chaperonin GroEL complexes encapsulating metal nano particles --Research works accomplished by using Electron Microscope System: XXV(2)--","subitem_title_language":"en"}]},"item_type_id":"10002","owner":"4","path":["129"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2020-11-24"},"publish_date":"2020-11-24","publish_status":"0","recid":"1122","relation_version_is_last":true,"title":["金属ナノ粒子内包 GroEL 複合体整列の検討 -- 分析電子顕微鏡システム利用研究成果、そのXXV(2) --"],"weko_creator_id":"4","weko_shared_id":-1},"updated":"2023-08-04T09:26:06.949127+00:00"}