| Item type |
紀要論文 / Departmental Bulletin Paper(1) |
| 公開日 |
2020-11-24 |
| タイトル |
|
|
タイトル |
組換えβ-secretase産生系の構築とセクレターゼ活性測定法への応用 |
|
言語 |
ja |
| タイトル |
|
|
タイトル |
Construction of recombinant beta-secretase production system and the application to measurement of the activity |
|
言語 |
en |
| 言語 |
|
|
言語 |
jpn |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Alzheimer disease |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
β-secretase |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
immobilized enzyme |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Flow system |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
departmental bulletin paper |
| ID登録 |
|
|
ID登録 |
10.34411/00001108 |
|
ID登録タイプ |
JaLC |
| 著者 |
白石, 有希
小池, あゆみ
山村, 晃
飯田, 泰広
Shiraishi, Yuki
Koike-Takeshita, Ayumi
Yamamura, Akira
Iida, Yasuhiro
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Alzheimer disease (AD) is the most common form of dementia. We does not achieved this disease fundamental cure. In AD case, the insoluble amyloid is produced by β and γ secretase by catalyzing the amyloid precursor peptide. Therefore, those enzymes are regarded as the primary target of pharmaceutical development of AD. All the β-secretase inhibitors are substrate analogs, it means that those are competitive and reversible inhibitor. We have developed new methods which can evaluate irreversible enzyme inhibitors easily by flow injection analysis (FIA) system in combination with immobilized enzyme and we would like to apply the β- secretase to the system for evaluation of irreversible agents. In this research, we tried to construct an expression vector for β-secretase and to investigate the optimum condition for obtaining the recombinant β-secretase activity. The β-secretase gene was cloned from pFN21A vector. Those fragment was subcloned into a pCold vector. Recombinant β-secretase was expressed by E.coli. |
|
言語 |
en |
| 書誌情報 |
神奈川工科大学研究報告.B,理工学編
巻 38,
p. 79-84,
発行日 2014-03-20
|
| 出版者 |
|
|
出版者 |
神奈川工科大学 |
| ISSN |
|
|
収録物識別子タイプ |
PISSN |
|
収録物識別子 |
21882878 |
| 書誌レコードID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA12669200 |
| フォーマット |
|
|
内容記述タイプ |
Other |
|
内容記述 |
application/pdf |
| 著者版フラグ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
kkb-038-013.pdf (1.3 MB)
