| Item type |
紀要論文 / Departmental Bulletin Paper(1) |
| 公開日 |
2020-11-24 |
| タイトル |
|
|
タイトル |
Survivin 相互作用を標的としたアポトーシス誘導剤スクリーニング系の開発 |
|
言語 |
ja |
| タイトル |
|
|
タイトル |
Development of screening system of apoptosis-inducing agent based on the survivin-interaction as a target. |
|
言語 |
en |
| 言語 |
|
|
言語 |
jpn |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
survivin |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
apoptosis |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
BIR domain |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
departmental bulletin paper |
| ID登録 |
|
|
ID登録 |
10.34411/00001125 |
|
ID登録タイプ |
JaLC |
| 著者 |
齋藤, 宇伸
長谷部, 佑亮
前澤, 祐花
高村, 岳樹
飯田, 泰広
Saitoh, Takanobu
Hasebe, Yusuke
Maezawa, Yuka
Takamura, Takeji
Iida, Yasuhiro
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Apoptosis is necessary to keep homeostasis for the human. Survivin is a member of the Inhibitor of Apoptosis Protein (IAP) family. If DNA is damaged in cells, the DNA will be repaired. However, if the irreparable damage occurs, the cells will induce apoptosis. It is known that the apoptosis is suppressed by inhibition of caspase-9 activity by binding of survivin to the caspase-9, and that it is needed to form a complex o f survivin with XIAP to inhibit the activity of caspase-9. Therefore, inhibition of interaction of survivin and XIAP will become a target of apoptosis induction. In this study, we developed an evaluation system of interaction of survivin and XIAP based on a Two-Hybrid method. Survivin and XIAP were cloned from HepG2 cells and those were constructed as vectors pACTII-survivin (prey vector) and pAS1-XIAP (bait vector). The yeast was transformed with both vectors by lithium acetate method and cultured in SG medium including the X -gal. In the results, blue colony attributed to survivin and XIAP interaction was obtained and the results indicated that the evaluation system of survivin-XIAP interaction was successfully developed. |
|
言語 |
en |
| 書誌情報 |
神奈川工科大学研究報告.B,理工学編
巻 39,
p. 73-80,
発行日 2015-03-20
|
| 出版者 |
|
|
出版者 |
神奈川工科大学 |
| ISSN |
|
|
収録物識別子タイプ |
PISSN |
|
収録物識別子 |
21882878 |
| 書誌レコードID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA12669200 |
| フォーマット |
|
|
内容記述タイプ |
Other |
|
内容記述 |
application/pdf |
| 著者版フラグ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
kkb-039-013.pdf (1.1 MB)
