| Item type |
紀要論文 / Departmental Bulletin Paper(1) |
| 公開日 |
2021-05-10 |
| タイトル |
|
|
タイトル |
Asp73とAsp420は酵母ミトコンドリアHsp60のATP加水分解に重要な役割を果たす --材料分析室利用研究成果、そのXXXI(1)-- |
|
言語 |
ja |
| タイトル |
|
|
タイトル |
Asp73 and Asp420 play a critical role in ATP hydrolysis of yeast mitochondrial Hsp60 --Research works accomplished by using materials analysis facilities: XXXI(1)-- |
|
言語 |
en |
| 言語 |
|
|
言語 |
jpn |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
GroEL |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Hsp60 |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Chaperonin |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
ATP hydrolysis |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
departmental bulletin paper |
| ID登録 |
|
|
ID登録 |
10.34411/00032044 |
|
ID登録タイプ |
JaLC |
| 著者 |
増田, 恵
村越, のどか
依田, ひろみ
小池, あゆみ
Masuda, Megumi
Murakoshi, Nodoka
Yoda, Hiromi
Koike-Takeshita, Ayumi
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Type I chaperonins (Cpn60/Hsp60) are essential proteins that mediate the correct folding of newly translated and stress-denatured proteins in bacteria, chloroplast and mitochondria. Despite the high sequence homology among chaperonins, there are some critical differences at the mechanistic level between the bacterial and the mitochondrial chaperonins with respect to their oligomeric state and their ability to function with co-chaperonins from different sources. Asp52 and Asp398 in Escherichia coli chaperonin GroEL are known as the critical residues on ATP hydrolysis because GroELD52A/D398A mutant is severely deficient in ATP hydrolysis but not in ATP binding. Here, yeast mitochondria Hsp60 (mHsp60) and Thermus thermophilus Hsp60 (TCpn60) were used to investigate the functional commonality of these two well-conserved Asp in ATP hydrolysis. These residues in GroEL correspond to Asp73 and Asp420 in mHsp60 and to Asp51 and Asp396 in TCpn60, respectively. We found that ATPase activity of mHsp60D73A and mHsp60D420A mutants were ~20% and < 4% of wild type, respectively, indicating that functional commonality of two Asp was shown in yeast mHsp60, despite suggesting different roles of Asp51 in TCpn60, surprisingly. |
|
言語 |
en |
| 書誌情報 |
神奈川工科大学研究報告.B,理工学編
巻 45,
p. 27-33,
発行日 2021-03-01
|
| 出版者 |
|
|
出版者 |
神奈川工科大学 |
| ISSN |
|
|
収録物識別子タイプ |
PISSN |
|
収録物識別子 |
21882878 |
| 書誌レコードID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA12669200 |
| フォーマット |
|
|
内容記述タイプ |
Other |
|
内容記述 |
application/pdf |
| 著者版フラグ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
kkb-045-004.pdf (1.5 MB)
