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Saccharomyces cerevisiaeを用いた小胞輸送阻害物質探索系の構築と生薬スクリーニング
https://doi.org/10.34411/00032046
https://doi.org/10.34411/00032046bc946e22-6c4d-4fbc-8a09-d2a0b25555f1
| 名前 / ファイル | ライセンス | アクション |
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kkb-045-006.pdf (641.1 kB)
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| Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||||||||||
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| 公開日 | 2021-05-10 | |||||||||||||
| タイトル | ||||||||||||||
| タイトル | Saccharomyces cerevisiaeを用いた小胞輸送阻害物質探索系の構築と生薬スクリーニング | |||||||||||||
| 言語 | ja | |||||||||||||
| タイトル | ||||||||||||||
| タイトル | Development of a novel evaluation system of tip growth for antifungal compounds with use of S. cerevisiae and application of the system for the screening in herbal medicine. | |||||||||||||
| 言語 | en | |||||||||||||
| 言語 | ||||||||||||||
| 言語 | jpn | |||||||||||||
| キーワード | ||||||||||||||
| 言語 | en | |||||||||||||
| 主題Scheme | Other | |||||||||||||
| 主題 | antifungal agent | |||||||||||||
| キーワード | ||||||||||||||
| 言語 | en | |||||||||||||
| 主題Scheme | Other | |||||||||||||
| 主題 | drug screening | |||||||||||||
| キーワード | ||||||||||||||
| 言語 | en | |||||||||||||
| 主題Scheme | Other | |||||||||||||
| 主題 | tip growth | |||||||||||||
| 資源タイプ | ||||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||
| 資源タイプ | departmental bulletin paper | |||||||||||||
| ID登録 | ||||||||||||||
| ID登録 | 10.34411/00032046 | |||||||||||||
| ID登録タイプ | JaLC | |||||||||||||
| 著者 |
小山, 菜穂
× 小山, 菜穂
× 飯田, 泰広
× Koyama, Naho
× Iida, Yasuhiro
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| 抄録 | ||||||||||||||
| 内容記述タイプ | Abstract | |||||||||||||
| 内容記述 | The infection by fungus such as yeast or mold is called mycoses. Among mycoses, deep mycoses are most likely to have more severe symptoms. To cure those deep mycoses, antifungal agents is used as a chemotherapy. However, the number of antifungal agents which can be used for treatment are only 4 classes 11 types. In addition, side effects and the appearance of resistant fungi have been confirmed for these drugs. Therefore, it is necessary to develop an antifungal agent different from the conventional antifungal agent. Thus, we focused on a tip growth, which is a unique growth system of fungi. Tip growth is a method of growth by repeating the decomposition and the resynthesis of the cell wall. It has been reported that the cell wall-degrading enzyme β-1,3-D-Glucanase (BGL2) involved in this tip growth is vesicular transported from the inside of the cell to the growth point. We consider that targeting vesicle transport during tip growth will provide selective toxicity to fungi. The purpose of this study is to develop novel antifungal agents targeting vesicle transport of fungal tip growth. Therefore, we constructed an evaluation system for visualizing vesicle transport using Saccharomyces cerevisiae. This evaluation system can visualize the vesicle transport of BGL2 during tip growth by fusion of the green fluorescent protein (EmGFP) to BGL2. The constructed tip growth evaluation systems were applied to search for vesicle transport inhibitor in herbal medicine. As a result, vesicle transport inhibitory activity was observed in 8 Kinds of herbal medicine extract. |
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| 言語 | en | |||||||||||||
| 書誌情報 |
神奈川工科大学研究報告.B,理工学編 巻 45, p. 41-47, 発行日 2021-03-01 |
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| 出版者 | ||||||||||||||
| 出版者 | 神奈川工科大学 | |||||||||||||
| ISSN | ||||||||||||||
| 収録物識別子タイプ | PISSN | |||||||||||||
| 収録物識別子 | 21882878 | |||||||||||||
| 書誌レコードID | ||||||||||||||
| 収録物識別子タイプ | NCID | |||||||||||||
| 収録物識別子 | AA12669200 | |||||||||||||
| フォーマット | ||||||||||||||
| 内容記述タイプ | Other | |||||||||||||
| 内容記述 | application/pdf | |||||||||||||
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| 出版タイプ | VoR | |||||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||||
