| Item type |
紀要論文 / Departmental Bulletin Paper(1) |
| 公開日 |
2021-05-10 |
| タイトル |
|
|
タイトル |
組換え維持型メチル化酵素を用いた新規メチル化解析法 |
|
言語 |
ja |
| タイトル |
|
|
タイトル |
Novel methylation analysis method with use of recombinant maintenance methyltransferase |
|
言語 |
en |
| 言語 |
|
|
言語 |
jpn |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Epigenetics |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
DNA methylation |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Bisulfite sequencing method |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
DNA (cytosine-5) methyltransferase 1 |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
departmental bulletin paper |
| ID登録 |
|
|
ID登録 |
10.34411/00032048 |
|
ID登録タイプ |
JaLC |
| 著者 |
吉川, 僚汰
前田, 翔大
髙村, 岳樹
飯田, 泰広
Yoshikawa, Ryota
Maeda, Shota
Takamura, Takeji
Iida, Yasuhiro
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
DNA methylation is one of epigenetics, and aberrant DNA methylation is related to various diseases such as cancer, neurological diseases, and allergic diseases. It is necessary to understand the methylation status of DNA in order to elucidate the fundamental cause of these diseases and to make an accurate diagnosis. Currently, the bisulfite sequencing method (BS-seq) is generally widely used as a DNA methylation analysis method. However, BS-seq has a problem that it cannot distinguish between hydroxymethylated cytosine (hmC) and methylated cytosine (mC).
Therefore, our laboratory is trying to develop a new methylation analysis method that combines DNA (cytosine-5) methyltransferase 1 (Dnmt1) and Polymerase.
In this study, recombinant Dnmt1 with low de novo activity and low activity against hmC in vitro was prepared and applied to the methylation analysis of the tyrosinase promoter of B16 melanoma. As a result, the difference in the methylation site was observed between the analysis result of only BS-seq and the analysis result of the novel methylation analysis method.
From this result, it is considered that by combining BS-seq with recombinant Dnmt1, only methylation could be detected excluding hydroxymethylation. |
|
言語 |
en |
| 書誌情報 |
神奈川工科大学研究報告.B,理工学編
巻 45,
p. 57-65,
発行日 2021-03-01
|
| 出版者 |
|
|
出版者 |
神奈川工科大学 |
| ISSN |
|
|
収録物識別子タイプ |
PISSN |
|
収録物識別子 |
21882878 |
| 書誌レコードID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA12669200 |
| フォーマット |
|
|
内容記述タイプ |
Other |
|
内容記述 |
application/pdf |
| 著者版フラグ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
kkb-045-008.pdf (1.4 MB)
