| Item type |
紀要論文 / Departmental Bulletin Paper(1) |
| 公開日 |
2022-04-28 |
| タイトル |
|
|
タイトル |
アポトーシス誘導性抗腫瘍物質スクリーニング用の酵母ツーハイブリッドシステムの開発 |
|
言語 |
ja |
| タイトル |
|
|
タイトル |
Development of Yeast Two Hybrid system for screening of apoptosis-inducing anti-tumor reagent |
|
言語 |
en |
| 言語 |
|
|
言語 |
jpn |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Apoptosis |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Survivin |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
HBXIP |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
BIR domain |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
departmental bulletin paper |
| ID登録 |
|
|
ID登録 |
10.34411/00032063 |
|
ID登録タイプ |
JaLC |
| 著者 |
秋山, 佳穂
長谷部, 佑亮
齋藤, 宇伸
髙村, 岳樹
飯田, 泰広
Akiyama, Yoshiho
Hasebe, Yusuke
Saito, Takanobu
Takamura, Takeji
Iida, Yasuhiro
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Apoptosis is a type of death that occur in multicellular organisms as a programmed cell death, and it is necessary to keep an individual development and homeostasis. If DNA is damaged in cells, the DNA will be repaired. However, if the irreparable damage occurs, the cells will induce apoptosis. One of the basic characteristics of cancer is having resistance to apoptosis, and the function of the apoptosis is suppressed in many kinds of cancer cells. Survivin is a member of the Inhibitor of Apoptosis Protein (IAP) family. It is hardly expressed in normal cells but is known to be highly expressed in many cancer cells. It is also known that Survivin interacts with HBXIP (Hepatitis B X-interacting protein) and the complex inhibit apoptosis in many tumor cells. Therefore, inhibition of interaction of Survivin and HBXIP will become a new target of apoptosis induction to tumor cells.
In this study, we developed an evaluation system of interaction of Survivin-α and HBXIP based on a Two-Hybrid method. Survivin-α and HBXIP were respectively inserted into the pACTII and pAS1 vector, and the two vecters were introduce into Saccharomyces cerevisiae BY 3913. Growth of transformed yeast in 3-AT assey suggested that two vectors were introduced into the yeast and that Survivin and HBXIP were interacted. In addition, we developed an evaluation method of Survivin-HBXIP interaction by β-galactosidase activity of the Two-Hybrid system. Our method will be applicable to screening method for apoptosis inducer against tumor cells. |
|
言語 |
en |
| 書誌情報 |
神奈川工科大学研究報告.B,理工学編
巻 46,
p. 41-48,
発行日 2022-03-01
|
| 出版者 |
|
|
出版者 |
神奈川工科大学 |
| ISSN |
|
|
収録物識別子タイプ |
PISSN |
|
収録物識別子 |
21882878 |
| 書誌レコードID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA12669200 |
| フォーマット |
|
|
内容記述タイプ |
Other |
|
内容記述 |
application/pdf |
| 著者版フラグ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
kkb-046-006.pdf (1.6 MB)
