| Item type |
紀要論文 / Departmental Bulletin Paper(1) |
| 公開日 |
2023-04-17 |
| タイトル |
|
|
タイトル |
高等学校の授業実態に即した分子生物学実験の実践と考察 |
|
言語 |
ja |
| タイトル |
|
|
タイトル |
Practical Consideration of Molecular Biological Education Suited for Japanese Highschools |
|
言語 |
en |
| 言語 |
|
|
言語 |
jpn |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
gap-bridging between high school and university |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
molecular biological experiments |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
DNA extraction |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
DNA quantification |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
PCR |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
agarose electrophoresis |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
departmental bulletin paper |
| ID登録 |
|
|
ID登録 |
10.34411/00032069 |
|
ID登録タイプ |
JaLC |
| 著者 |
針谷, 桃華
橋場, 美穂
甲斐, 由理子
押鐘, 浩之
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Whilst molecular biological articles have been extensively described in high school-level textbook guidelined by MEXT, it is, however, commonly deemed to be practically difficult to perform molecular biological experiments in high schools. We herein report an ingenious protocol for molecular biological experiments best suitable for biology classes in Japanese high schools, comprising DNA extraction, DNA detection, PCR and agarose electrophoresis, all of which can be completed substantially within no more than two classes. In order to exploit the protocol, we cautiously cared the following points: sets of the experiments can be accomplished in typical period in high schools (50 minutes); and most of the listed experiments can be performed in standard high school facilities. For DNA extraction, “silica monolith” spin column (Animos Co. Ltd.) was employed, much facilitating and shortening the procedure (completed within ~10 minutes). DNA from rice powder (O. sativa) and flour (T. aestivum) was successfully detected by means of agarose electrophoresis, followed by PCR amplification by Taq polymerase reaction mixture (Quick Taq® HS DyeMix; Toyobo Co. Ltd.), targeted for RuBisCO small subunit gene through O. sativa specific primers. The amplification was confirmed by agarose electrophoresis, non-hazardously stained by Midori Green (Nippon Genetics Co. Ltd.). To further develop the experimental protocol, it is thought to be necessary not only to supply the equipment required for routine molecular biological experiments in the high schools, but also to manufacture either illustrated or visualised protocol, expecting for future facilitation of molecular biological learning in high schools. |
|
言語 |
en |
| 書誌情報 |
神奈川工科大学研究報告.A,人文社会科学編
巻 47,
p. 29-37,
発行日 2023-03-01
|
| 出版者 |
|
|
出版者 |
神奈川工科大学 |
| ISSN |
|
|
収録物識別子タイプ |
PISSN |
|
収録物識別子 |
21882878 |
| 書誌レコードID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA12669200 |
| フォーマット |
|
|
内容記述タイプ |
Other |
|
内容記述 |
application/pdf |
| 著者版フラグ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
kka-047-004.pdf (1.5 MB)
